Different phosphorylated forms of RNA polymerase II and associated mRNA processing factors during transcription

• DOI: 10.1101/gad.824700
• Publication Date: 2002-07-26T23:59:15Z
• Authors: Philip Komarnitsky; Eun-Jung Cho; Stephen Buratowski
• Keywords: RNA polymerase II; Transcription; General transcription factor; Chromatin immunoprecipitation

The activities of several mRNA processing factors are coupled to transcription through binding RNA polymerase II (Pol II). largest subunit Pol contains a repetitive carboxy-terminal domain (CTD) that becomes highly phosphorylated during transcription. mRNA-capping enzyme binds only CTD, whereas other may bind both and unphosphorylated forms. Capping occurs soon after initiation before events, raising the question whether capping components remain associated with complex they have modified 5′ end mRNA. Chromatin immunoprecipitation in Saccharomyces cerevisiae shows cross-links promoters but not coding regions. In contrast, cap methyltransferase Hrp1/CFIB polyadenylation factor cross-link promoter Remarkably, phosphorylation pattern CTD changes Ser 5 is detected primarily at regions dependent on TFIIH. 2 seen These results suggest dynamic association differently forms throughout cycle.