Novel roles for KLF1 in erythropoiesis revealed by mRNA-seq

• DOI: 10.1101/gr.135707.111
• Publication Date: 2012-07-27T20:32:14Z
• Authors: Michael R. Tallack; Graham W. Magor; Benjamin Dartigues; Lei Sun; Stephen Huang; Jessica M. Fittock; Sally V. Fry; Evgeny A. Glazov; Timothy L. Bailey; Andrew C. Perkins
• Keywords: GATA1

KLF1 (formerly known as EKLF) regulates the development of erythroid cells from bi-potent progenitor via transcriptional activation a diverse set genes. Mice lacking Klf1 die in utero prior to E15 severe anemia due inadequate expression genes controlling hemoglobin production, cell membrane and cytoskeletal integrity, cycle. We have recently described full repertoire binding sites vivo by performing ChIP-seq primary tissue (E14.5 fetal liver). Here we describe KLF1-dependent transcriptome comparing mRNA-seq Klf1(+/+) Klf1(-/-) tissue. This has revealed novel target not previously obtainable traditional microarray technology, provided insights into function activator. define cis-regulatory module bound KLF1, GATA1, TAL1, EP300 that coordinates core also erythroid-specific promoters drive high-level otherwise ubiquitously expressed cells. Our study identified two lncRNAs are dynamically during differentiation, discovered role for directing apoptotic gene terminal stages maturation.