Genome-wide phosphoacetylation of histone H3 at Drosophila enhancers and promoters

• DOI: 10.1101/gr.136929.111
• Publication Date: 2012-04-17T04:30:24Z
• Authors: Wendy A. Kellner; Edward Ramos; Kevin Van Bortle; Naomi Takenaka; Victor G. Corces
• Keywords: Ecdysone receptor; Enhancer RNAs; Transcription

Transcription regulation is mediated by enhancers that bind sequence-specific transcription factors, which in turn interact with the promoters of genes they control. Here, we show JIL-1 kinase present at both and ecdysone-induced Drosophila genes, where it phosphorylates Ser10 Ser28 residues histone H3. also required for CREB binding protein (CBP)-mediated acetylation Lys27, a well-characterized mark active enhancers. The presence these proteins results establishment H3K9acS10ph H3K27acS28ph marks regulatory sequences. These modifications are necessary recruitment 14-3-3, scaffolding capable facilitating interactions between two simultaneously bound proteins. Chromatin conformation capture assays indicate interaction enhancer promoter dependent on JIL-1, CBP. Genome-wide analyses extend conclusions to most showing H3K9acS10ph, general feature this organism.