H3K4me1 marks DNA regions hypomethylated during aging in human stem and differentiated cells

• DOI: 10.1101/gr.169011.113
• Publication Date: 2014-10-01T08:45:46Z
• Authors: Agustín F. Fernández; Gustavo F. Bayón; Rocío G. Urdinguio; Estela G. Toraño; María G. García; Antonella Carella; Sandra Petrus-Reurer; Cecilia Ferrero; Pablo Martinez-Ca...; Isabel Cubillo; Javier García-Castro; Jesús Delgado-Calle; Flor M. Pérez-Campo; José A. Riancho; Clara Bueno; Pablo Menéndez; Anouk Mentink; Katia Mareschi; Fabian Claire; Corrado Fagnani; Emanuela Medda; Virgilia Toccaceli; Sonia Brescianini; Sebastián Moran; Manel Esteller; Alexandra Stolzing; Jan de Boer; Lorenza Nisticò; Maria A. Stazi; Mario F. Fraga
• Keywords: Epigenomics; CpG site; Differentially methylated regions

In differentiated cells, aging is associated with hypermethylation of DNA regions enriched in repressive histone post-translational modifications. However, the chromatin marks changes methylation adult stem cells during lifetime are still largely unknown. Here, profiling mesenchymal (MSCs) obtained from individuals aged 2 to 92 yr identified 18,735 hypermethylated and 45,407 hypomethylated CpG sites aging. As sequences were marks. Most importantly, strongly active mark H3K4me1 suggesting this a cell type-independent signature hypomethylation Analysis scedasticity showed that interindividual variability increased MSCs providing new avenue for identification over time. genetically identical both tendency depended on nongenetic as well genetic factors. Our results indicate dynamics depend complex mixture factors include sequence, type, context involved that, depending locus, can be modulated by and/or external