Inherited DNA methylation primes the establishment of accessible chromatin during genome activation

• DOI: 10.1101/gr.228833.117
• Publication Date: 2018-05-29T18:45:13Z
• Authors: Guifen Liu; Wen Wang; Shengen Hu; Xiangxiu Wang; Yong Zhang
• Keywords: Epigenomics; ChIA-PET

For animals, epigenetic modifications can be globally or partially inherited from gametes after fertilization, and such information is required for proper transcriptional regulation, especially during the process of zygotic genome activation (ZGA). However, mechanism underlying how signatures affect regulation ZGA remains poorly understood. Here, we performed genome-wide profiling chromatin accessibility zebrafish ZGA, which closely related to regulation. We observed a clear trend toward gradual increase in accessible ZGA. Furthermore, at promoters displayed sequential priority emergence, locations were precisely primed by enrichment unmethylated CpGs that fully gametes. On other hand, distal regions with high methylation levels sperm facilitated binding DNA methylation-preferred transcription factors, as Pou5f3 Nanog, contributed establishment these loci. Our results demonstrate model whereby prime through two distinct mechanisms.