Chromosome Conformation Capture Carbon Copy (5C): A massively parallel solution for mapping interactions between genomic elements

• DOI: 10.1101/gr.5571506
• Publication Date: 2006-09-06T03:45:38Z
• Authors: Josée Dostie; Todd A. Richmond; Ramy A. Arnaout; Rebecca R. Selzer; William L. Lee; Tracey A. Honan; Eric D. Rubio; Anton Krumm; Justin Lamb; Chad Nusbaum; Roland D. Green; Job Dekker
• Keywords: Chromosome conformation capture

Physical interactions between genetic elements located throughout the genome play important roles in gene regulation and can be identified with Chromosome Conformation Capture (3C) methodology. 3C converts physical chromatin into specific ligation products, which are quantified individually by PCR. Here we present a high-throughput approach, 3C-Carbon Copy (5C), that employs microarrays or quantitative DNA sequencing using 454-technology as detection methods. We applied 5C to analyze 400-kb region containing human β-globin locus 100-kb conserved desert region. validated of several previously looping locus. also new interaction K562 cells Locus Control Region γ–β-globin intergenic Interestingly, this has been implicated control developmental globin switching. should widely applicable for large-scale mapping cis - trans networks genomic study higher-order chromosome structure.