Isolation and mapping of novel mouse brain cDNA clones containing trinucleotide repeats, and demonstration of novel alleles in recombinant inbred strains.

• DOI: 10.1101/gr.6.8.715
• Publication Date: 2007-06-05T21:18:07Z
• Authors: D M Chambers; C M Abbott
• Keywords: Direct repeat; Inbred strain; Homology

Abnormal expansion of trinucleotide repeats (TRs) has now been implicated in the pathogenesis at least nine human genetic disorders, particularly those which anticipation and/or fragile sites have demonstrated. Anticipation, phenomenon increasing severity phenotype successive generations, never seen species other than man. Nevertheless, animal models for dynamic mutation TRs would be extremely valuable. We screened a mouse brain cDNA library an attempt to identify clones representing each 10 possible classes repeat. Thirty-seven were analyzed detail. Of 37 sequences, 18 displayed significant levels homology with sequences GenBank, them expressed sequence tags (ESTs). then 25 by PCR containing repeat number different strains and assess variability length. this way, 64% showed length variation between Mus musculus spp. spretus, 32% musculus-derived standard laboratory inbred strains. Where was detected (17 repeat-containing all), gene mapped linkage analysis. None isolated any signs extreme expansion. However, two shown undergone size changes during establishment recombinant strains, suggesting that these are moderately unstable.