Complete Genomic Sequence and Analysis of the Prion Protein Gene Region from Three Mammalian Species

• DOI: 10.1101/gr.8.10.1022
• Publication Date: 2017-05-03T00:28:30Z
• Authors: Inyoul Y. Lee; David Westaway; Arian F.A. Smit; Kai Wang; Jason Seto; Lei Chen; Chetana Acharya; Mike Ankener; Dale Baskin; Carol Cooper; Hong Yao; Stanley B. Prusiner; Leroy E. Hood
• Keywords: Cosmid; Coding region; Genomic Organization; genomic DNA

The prion protein (PrP), first identified in scrapie-infected rodents, is encoded by a single exon of single-copy chromosomal gene. In addition to the protein-coding exon, PrP genes mammals contain one or two 5′-noncoding exons. To learn more about genomic organization regions surrounding exons, we sequenced 10 5 bp DNA from clones containing human, sheep, and mouse isolated cosmids λ phage. Our findings are as follows: (1) Although human transcript does not include untranslated 2 found its sheep counterparts, large intron gene contains an 2-like sequence flanked consensus splice acceptor donor sites. (2) Prnp but b allele 44 inbred lines 6593 nucleotide retroviral genome inserted into anticoding strand 2. This intracisternal A-particle element duplications AAGGCT motif. (3) We that 40% 57% genome-wide repetitive elements independently increased size locus all three species numerous mutations. unusually long 3′-untranslated region “fossil” 1.2-kb mariner transposable element. (4) sequences noncoding conserved between may represent biologically functional [The data reported this paper have been submitted GenBank database assigned accession numbers U29185 (human), U29186 (mouse), U67922 (sheep).]