Brain region-specific effects of cGMP-dependent kinase II knockout on AMPA receptor trafficking and animal behavior

• DOI: 10.1101/lm.042960.116
• Publication Date: 2016-07-16T02:43:35Z
• Authors: Seonil Kim; Joseph E. Pick; Sinedu Abera; Latika Khatri; Danielle D.P. Fer...; Matheus F. Sathler; Sage L. Morison; Franz Hofmann; Edward B. Ziff
• Keywords: Male; 0301 basic medicine; Behavior, Animal; Depression; Research; Conditioning, Classical; Brain; Prefrontal Cortex; Cyclic GMP-Dependent Protein Kinase Type II; Fear; Amygdala; Hippocampus; Olfactory Bulb; Mice, Inbred C57BL; Smell; Protein Transport; 03 medical and health sciences; Animals; Receptors, AMPA; Phosphorylation

Phosphorylation of GluA1, a subunit AMPA receptors (AMPARs), is critical for AMPAR synaptic trafficking and control transmission. cGMP-dependent protein kinase II (cGKII) mediates this phosphorylation, cGKII knockout (KO) affects GluA1 phosphorylation alters animal behavior. Notably, in the KO hippocampus increased as functional compensation gene deletion, while such absent prefrontal cortex. Thus, there are brain region-specific effects on trafficking, which could affect Here, we show that levels differ various regions, specific behaviors altered according to changes phosphorylation. Moreover, identified distinct regulations phosphatases different leading regional heterogeneity brain. Our work demonstrates mice corresponding cognitive performance. We also reveal regulation region arise can selectively alter