Reentrant Liquid-Liquid Phase Separation in Protein Solutions at Elevated Hydrostatic Pressures

• DOI: 10.1103/physrevlett.112.028101
• Publication Date: 2014-01-21T19:27:01Z
• Authors: Johannes Möller; Sebastian Grobelny; Julian Schulze; Steffen Bieder; Andre Steffen; Mirko Erlkamp; Michael Paulus; Metin Tolan; Roland Winter
• Keywords: Reentrancy; Hydrostatic pressure; Liquid liquid; Hydrostatic equilibrium

We present results from small-angle x-ray scattering data on the effect of high pressure phase behavior dense lysozyme solutions in liquid-liquid separation region, and characterize underlying intermolecular protein-protein interactions as a function temperature this region space. A reentrant has been discovered at elevated pressures, which originates dependence solvent-mediated interactions.