Tyrosine and Phenylalanine Are Synthesized within the Plastids in Arabidopsis

• DOI: 10.1104/pp.108.130070
• Publication Date: 2009-01-10T01:49:04Z
• Authors: Pascal Rippert; Juliette Puyaubert; Delphine Grisollet; Laure Derrier; Michel Matringe
• Keywords: 0301 basic medicine; 570; Arabidopsis thaliana; Transcription, Genetic; shikimate; Phenylalanine; [SDV.BBM]Life Sciences [q-bio]/Biochemistry; Blotting, Western; Arabidopsis; chorismate mutase; plant; phenylalanine biosynthesis; 03 medical and health sciences; chloroplast; Gene Expression Regulation, Plant; subcellular localization; [SDV.BV]Life Sciences [q-bio]/Vegetal Biology; [SDV.BBM]Life Sciences [q-bio]/Biochemistry, Molecular Biology; arogenate dehydrogenase; Plastids; RNA, Messenger; Molecular Biology; quantitative RT-PCR; arogenate dehydratase; 580; Arabidopsis Proteins; Reverse Transcriptase Polymerase Chain Reaction; Gene Expression Profiling; metabolic pathway; tyrosine biosynthesis; Isoenzymes; Plant Leaves; aromatic aminoacid; enzyme; Protein Transport; gene expression; Tyrosine; metabolism; Subcellular Fractions

Abstract While the presence of a complete shikimate pathway within plant plastids is definitively established, existence cytosolic postchorismate portion still debated. This question alimented by chorismate mutase (CM) cytosol. Until now, only known destiny prephenate, product CM, incorporation into tyrosine (Tyr) and/or phenylalanine (Phe). Therefore, CM suggests that enzymes involved downstream in Tyr or Phe biosynthesis could be present cytosol cells. It was thus particular interest to clarify subcellular localization arogenate dehydrogenases (TYRAs) and dehydratases (ADTs), which catalyze ultimate steps biosynthesis, respectively. The aim this study address Arabidopsis (Arabidopsis thaliana) analysis two TYRAAts six AtADTs. article excludes occurrence spliced TYRAAt1 transcript encoding TYRA protein. Transient expression analyses TYRA- ADT-green fluorescent protein fusions reveal proteins ADT are all targeted plastid. Accordingly, were both immunodetected chloroplast soluble fraction (stroma) Arabidopsis. No Taken together, our data exclude possibility synthesis cytosol, at least green leaves cultured