Chromoplast-Specific Carotenoid-Associated Protein Appears to Be Important for Enhanced Accumulation of Carotenoids inhp1Tomato Fruits

• DOI: 10.1104/pp.112.212191
• Publication Date: 2013-02-12T02:57:52Z
• Authors: Himabindu Vasuki ...; Rakesh Kumar; Rameshwar Sharma; Yellamaraju Sreel...
• Keywords: Chromoplast

Tomato (Solanum lycopersicum) high-pigment mutants with lesions in diverse loci such as DNA Damage-Binding Protein1 (high pigment1 [hp1]), Deetiolated1 (hp2), Zeaxanthin Epoxidase (hp3), and Intense pigment (Ip; gene product unknown) exhibit increased accumulation of fruit carotenoids coupled an increase chloroplast number size. However, little is known about the underlying mechanisms exaggerating carotenoid these mutants. A comparison proteome profiles from outer pericarp hp1 mutant wild-type (cv Ailsa Craig) fruits at different developmental stages revealed least 72 differentially expressed proteins during ripening. Hierarchical clustering grouped into three clusters. We found abundance chromoplast-specific carotenoid-associated protein (CHRC) red-ripe stage that also reflected its transcript level. Western blotting using CHRC polyclonal antibody bell pepper (Capsicum annuum) a 2-fold compared wild type. levels hp2 were to be similar hp1, whereas hp3 Ip showed intermediate those hp2, fruits. Both present isolated plastoglobules. Overall, our results suggest loss function DDB1, DET1, Epoxidase, up-regulates levels. Increase may contribute enhanced content by assisting sequestration stabilization carotenoids.