Coculture of Bifidobacterium bifidum G9‐1 With Butyrate‐Producing Bacteria Promotes Butyrate Production

• DOI: 10.1111/1348-0421.13224
• Publication Date: 2025-04-24T04:42:18Z
• Authors: Haruka Yokota; Yoshiki Tanaka; Hiroshi Ohno
• Keywords: Bifidobacterium bifidum; Faecalibacterium prausnitzii; Short-chain fatty acid; Bifidobacterium breve

ABSTRACT Supplementation with Bifidobacterium bifidum G9‐1 (BBG9‐1) has been established to enhance the production of butyrate, a short‐chain fatty acid (SCFA) known for its beneficial effects in alleviating constipation. We hypothesized that BBG9‐1 alters gut microbiota such bacteria produce butyric from lactate and acetate become more abundant. In this study, we sought determine whether promotes growth butyrate‐producing thereby enhances butyrate production. was cocultured different compare differences SCFA cocultures monocultures. indeed detected significant increases SCFAs compared Moreover, increased time‐dependent manner Faecalibacterium prausnitzii ID 6052 coculture. addition, initially until 16 h, followed by decline between 20 24 subsequent increase at 48 h. Comparatively, Anaerostipes caccae JCM 13470 T coculture peaked h declined thereafter, manner. contrast, lactate, acetate, Roseburia hominis 17582 These findings indicate utilizing BBG9‐1‐produced acetate. Thus, butyrate‐mediated physiological activity could be attributed an indirect enhancement