Membrane association of SadC enhances its diguanylate cyclase activity to control exopolysaccharides synthesis and biofilm formation in Pseudomonas aeruginosa

• DOI: 10.1111/1462-2920.13263
• Publication Date: 2016-03-05T16:06:08Z
• Authors: Bin Zhu; Cuilan Liu; Shiheng Liu; Hengjiang Cong; Yihuan Chen; Lichuan Gu; Luyan Z. Ma
• Keywords: Second messenger system

Cyclic diguanosine monophosphate (c-di-GMP) is one of the most important bacterial second messengers that controls many cellular functions including lifestyle switch between plankton and biofilm. Surface attachment defective (SadC) a diguanylate cyclase (DGC) involved in biosynthesis c-di-GMP Pseudomonas aeruginosa, an opportunistic pathogen can cause diverse infections. Here we report crystal structure GGDEF domain from SadC critical role trans-membrane (TM) with regard to biofilm formation, exopolysaccharide production motility. We showed over-expression P. aeruginosa PAO1 totally inhibited swimming motility significantly enhanced Psl. lacking TM domains (SadC300-487 ) could not localize on cytoplasmic membrane form cluster, lost ability inhibit twitching motility, attenuated activity promote Psl despite SadC300-487 was able catalyze synthesize vitro vivo. The has typical α-helix connected essential for DGC oligomers. Our data imply association promotes its by affecting formation active