Pisiferdiol restores the growth of a mutant yeast suffering from hyperactivated Ca2+signalling through calcineurin inhibition

• DOI: 10.1111/1567-1364.12003
• Publication Date: 2012-09-26T23:41:13Z
• Authors: Nobuhiro Aburai; Jun Yoshida; Miki Kobayashi; Masaki Mizunuma; Motoko Ohnishi; Ken-ichi Kimura
• Keywords: Growth inhibition

In the course of our screening program for a new inhibitor Ca(2+) signalling pathway using mutant yeast [Saccharomyces cerevisiae (zds1Δ erg3Δ pdr1Δ pdr3Δ)], mouse PP2Cα activator, pisiferdiol, isolated from Chamaecyparis pisifera, was found to alleviate signal-mediated growth inhibition. Pisiferdiol showed inhibition activity against mpk1Δ strain compared with cnb1Δ and induced Li(+) sensitivity wild-type strain, indicating that it suppresses calcineurin in yeast. However, ptc1Δ by pisiferdiol diminished. restored zds1Δ without immunophilins Fkb1p or Cph1p, pmc1Δ strain. It inhibited calcineurin-induced expression reporter gene assay decreased protein (Western blots) (Cnb1p) addition decrease Swe1p phosphorylation Cdc28p These results could suppress indirectly action through Ptc1p activation.