Yeast synthetic biology for the production of recombinant therapeutic proteins

• DOI: 10.1111/1567-1364.12195
• Publication Date: 2014-08-12T08:56:09Z
• Authors: Hyunah Kim; Su Jin Yoo; Hyun Ah Kang
• Keywords: Yarrowia; Synthetic Biology; Biopharmaceutical; Pichia

The production of recombinant therapeutic proteins is one the fast-growing areas molecular medicine and currently plays an important role in treatment several diseases. Yeasts are unicellular eukaryotic microbial host cells that offer unique advantages producing biopharmaceutical proteins. capable robust growth on simple media, readily accommodate genetic modifications, incorporate typical post-translational modifications. Saccharomyces cerevisiae a traditional baker's yeast has been used as major for biopharmaceuticals; however, nonconventional species including Hansenula polymorpha, Pichia pastoris, Yarrowia lipolytica have gained increasing attention alternative hosts industrial In this review, we address established emerging tools strains suitable protein various expression systems, particularly focusing current efforts toward synthetic biology approaches developing cell factories