Novel peptides from sea cucumber intestines hydrolyzed by neutral protease alleviate exercise‐induced fatigue via upregulating the glutaminemediated Ca2+/Calcineurin signaling pathway in mice

• DOI: 10.1111/1750-3841.16934
• Publication Date: 2024-01-23T10:56:23Z
• Authors: Xutong Lu; Meng Wang; Hao Yue; Xiaoxuan Feng; Yingying Tian; Changhu Xue; Tiantian Zhang; Yuming Wang
• Keywords: 0301 basic medicine; 0303 health sciences; Calcineurin; Sea Cucumbers; Intestines; Mice; Animals; Muscle, Skeletal; Peptides; Swimming; Signal Transduction; Peptide Hydrolases

Abstract Sea cucumber intestines are considered a valuable resource in the sea processing industry due to their balanced amino acid composition. Studies have reported that peptides rich glutamate and branched‐chain acids anti‐fatigue properties. However, function of intestine reducing exercise‐induced fatigue remains unclear. In this study, we enzymatically hydrolyzed low molecular weight from (SCIP) administered SCIP orally mice examine its effects on using swimming pole‐climbing exhaustion experiments. The results revealed supplementation with significantly prolonged time mice, decreased blood lactate urea nitrogen levels, increased liver muscle glycogen levels following weight‐loaded test. Immunofluorescence analysis indicated notable increase proportion slow‐twitch fiber significant decrease fast‐twitch supplementation. Furthermore, upregulated mRNA expression Ca 2+ /Calcineurin upstream downstream regulators, thereby contributing promotion skeletal type conversion. This study presents initial evidence establishing as potential enhancer resistance, consequently providing theoretical foundation for utilization intestines.