A chromosome‐level genome assembly ofPyropia haitanensis(Bangiales, Rhodophyta)

• DOI: 10.1111/1755-0998.13102
• Publication Date: 2019-10-10T21:39:20Z
• Authors: Min Cao; Kuipeng Xu; Xinzi Yu; Guiqi Bi; Yang Liu; Fanna Kong; Peipei Sun; Xianghai Tang; Guoying Du; Yuan Ge; Dongmei Wang; Yunxiang Mao
• Keywords: Genome size; Sequence assembly; Bacterial artificial chromosome; Illumina dye sequencing

Pyropia haitanensis (Bangiales, Rhodophyta), a major economically important marine crop, is also considered as an ideal research model of Rhodophyta to address several biological questions such sexual reproduction and adaptation intertidal abiotic stresses. However, comparative genomic analysis decipher the underlying molecular mechanisms hindered by lack high-quality genome information. Therefore, we integrated sequencing data from Illumina short-read sequencing, PacBio single-molecule BioNano optical mapping. The assembled was approximately 53.3 Mb with average GC% 67.9%. contig N50 scaffold were 510.3 kb 5.8 Mb, respectively. Additionally, 10 superscaffolds representing 80.9% total assembly (42.7 Mb) anchored orientated 5 linkage groups based on markers genetic distance; this outcome consistent karyotype five chromosomes (n = 5) cytological observation in P. haitanensis. Approximately 9.6% 14.6% region interspersed repeat tandem elements, Based full-length transcriptome generated PacBio, 10,903 protein-coding genes identified. construction genome-wide phylogenetic tree demonstrated that divergence time Porphyra umbilicalis ~204.4 Ma. Interspecies comparison revealed 493 gene families expanded 449 contracted compared those Po. genome. identified great value for further evolution red algae