Genetic structure and insecticide resistance characteristics of fall armyworm populations invading China

• DOI: 10.1111/1755-0998.13219
• Publication Date: 2020-07-03T18:20:45Z
• Authors: Lei Zhang; Bo Liu; Weigang Zheng; Conghui Liu; Dandan Zhang; Shengyuan Zhao; Zaiyuan Li; Pengjun Xu; Kenneth Wilson; Amy Withers; Christopher M. Jones; Judith A. Smith; Gilson Chipabika; Donald L. Kachigamba; Kiwoong Nam; Emmanuelle d’Alençon; Bei Liu; Xinyue Liang; Minghui Jin; Chao Wu; Swapan Chakrabarty; Xianming Yang; Yuying Jiang; Jie Liu; Xiaolin Liu; Weipeng Quan; Guirong Wang; Wei Fan; Wanqiang Qian; Kongming Wu; Yutao Xiao
• Keywords: Fall armyworm; Bacillus thuringiensis; Pesticide resistance

Abstract The rapid wide‐scale spread of fall armyworm ( Spodoptera frugiperda ) has caused serious crop losses globally. However, differences in the genetic background subpopulations and mechanisms adaptation behind invasion are still not well understood. Here we report assembly a 390.38‐Mb chromosome‐level genome derived from south‐central Africa using Pacific Bioscience (PacBio) Hi‐C sequencing technologies, with scaffold N50 12.9 Mb containing 22,260 annotated protein‐coding genes. Genome‐wide resequencing 103 samples strain identification were conducted to reveal populations China. Analysis genes related pesticide‐ Bacillus thuringiensis (Bt) resistance showed that risk developing conventional pesticides is very high. Laboratory bioassay results insects invading China carry organophosphate pyrethroid pesticides, but sensitive genetically modified maize expressing Bt toxin Cry1Ab field experiments. Additionally, two mitochondrial fragments found be inserted into nuclear genome, insertion event occurring after differentiation strains. This study represents valuable advance toward improving management strategies for armyworm.