Hydroxysafflor yellow A inhibited lipopolysaccharide‐induced non‐small cell lung cancer cell proliferation, migration, and invasion by suppressing the PI3K/AKT/mTOR and ERK/MAPK signaling pathways

• DOI: 10.1111/1759-7714.13019
• Publication Date: 2019-05-06T15:55:08Z
• Authors: Ming Jiang; Li‐Yang Zhou; Nan Xu; Qing An
• Keywords: non‐small cell lung cancer; Lipopolysaccharides; 0301 basic medicine; Lung Neoplasms; Hydroxysafflor yellow A; Phosphatidylinositol 3-Kinases; 03 medical and health sciences; Chalcone; Cell Movement; Carcinoma, Non-Small-Cell Lung; Cell Line, Tumor; PI3K/AKT/mTOR; Humans; Extracellular Signal-Regulated MAP Kinases; RC254-282; ERK/MAPK; Cell Proliferation; TOR Serine-Threonine Kinases; lipopolysaccharide; Quinones; Neoplasms. Tumors. Oncology. Including cancer and carcinogens; Original Articles; 3. Good health; Gene Expression Regulation, Neoplastic; A549 Cells; Proto-Oncogene Proteins c-akt; Signal Transduction

BackgroundChronic inflammation plays a significant role in the occurrence and development of non‐small cell lung cancer (NSCLC). Hydroxysafflor yellow A (HSYA), a chemical compound of the yellow color pigments extracted from the safflower, has been widely used in clinical treatment with positive antioxidation, anti‐inflammation, and antitumor effects. However, the role and underlying mechanisms of HYSA on development and progress in inflammation‐mediated NSCLC are unknown.MethodsCell counting kit‐8, colony formation, EdU, cell apoptosis, wound healing, Transwell migration and invasion, and enzyme‐linked immunosorbent assays; flow cytometry; and Western blotting were conducted using human NSCLC cell lines A549 and H1299.ResultsLipopolysaccharide (LPS) significantly promoted the proliferation and enhanced colony formation of A549 and H1299 cells, while HYSA notably reversed the effects of LPS. HYSA induced apoptosis of LPS‐mediated A549 and H1299 cells in a dose dependent manner; and remarkably suppressed migration, invasion, and epithelial–mesenchymal transition (EMT), significantly regulated production of LPS‐induced inflammation cytokines, and downregulated protein expression of PI3K/Akt/mTOR and ERK/MAPK signaling pathways in LPS‐induced A549 and H1299 cells. Furthermore, PI3K (LY294002) and ERK (SCH772984) inhibitors remarkably inhibited proliferation, migration, invasion, and EMT, and induced apoptosis in LPS‐mediated A549 and H1299 cells. These effects were even more obvious in the presence of HYSA and LY294002 or SCH772984 compared to those of either agent alone.ConclusionHYSA suppressed LPS‐mediated proliferation, migration, invasion, and EMT in A549 and H1299 cells by inhibiting the PI3K/Akt/mTOR and ERK/MAPK signaling pathways, indicating that HYSA may be a potential candidate to treat inflammation‐mediated NSCLC.