Sporisorium scitamineum colonisation of sugarcane genotypes susceptible and resistant to smut revealed by GFP‐tagged strains

• DOI: 10.1111/aab.12304
• Publication Date: 2016-07-27T06:31:46Z
• Authors: G. Carvalho; M.C. Quecine; D.P. Longatto; L.P. Peters; J.R. Almeida; T.G. Shyton; M.M.L. Silva; G.S. Crestana; S. Creste; C.B. Monteiro‐Vit...
• Keywords: Smut; Colonisation

Abstract Sporisorium scitamineum is the causal agent of sugarcane smut disease. The fungus establishes a biotrophic interaction with tissues, and unlike fungi other monocot hosts, primary meristem plants develops whip‐like structure instead tumour‐like galls emerging from floral structures (tassels ears). We examined (GFP)‐tagged S. infecting tissues three genotypes distinct responses to (susceptible, intermediate resistant resistant). Mating compatible haploid cells gfp ‐expressing were obtained by Agrobacterium tumefaciens ‐mediated transformation (ATMT) using integrative vector pFAT‐gfp. Regardless inoculation method (drop hypodermal syringe inoculation), all colonised fungus. GFP‐tagged strains opposite mating reaction able to: (a) grow in vitro as fluorescent yeast‐like cells; (b) generate infectious dikaryon; (c) penetrate tissues; (d) colonise growing filamentous network; (e) form characteristic highly branched hyphae within host cells. Fungal colonisation 160 DAI revealed an association vascular vessels disrupting their organisation analysed. However, did not develop whips spanning experiment time. first emerged 76 susceptible genotype whereas for detected at 137 DAI. These dissected sporogenesis teliospore maturation In germination recovered teliospores after meiosis formation three‐celled hyphal filament, where fourth cell was likely maintained coat. showed independent segregation marker, result insertions different chromosomes each strain. This work presents complete fungal life cycle GFP‐marked study developmental stages planta .