Salinity stress response of non‐transformed and AtCKX transgenic centaury (Centaurium erythraea Rafn.) shoots and roots grown in vitro

• DOI: 10.1111/aab.12593
• Publication Date: 2020-04-13T12:09:52Z
• Authors: Milana Trifunović...; Danijela Paunović; Snežana Milošević; Marija Marković; Slađana Jevremović; Ivana Č Dragićević; Angelina Subotić
• Keywords: Malondialdehyde

Abstract Common centaury ( Centaurium erythraea Rafn.) is a plant species that can inhabit saline soils. It known as with high spontaneous regeneration potential in vitro. In the present work we evaluated shoots and roots salinity tolerance of non‐transformed three AtCKX transgenic lines to graded NaCl concentrations (0, 50, 100, 150, 200 mM) Overexpression genes plants resulted an altered cytokinins (CKs) profile leading decline bioactive CK levels and, at same time, increased contents storage forms, inactive forms and/or nucleotides. Significant increment fresh shoot weight was obtained AtCKX1 line only on medium supplemented 50 mM NaCl. However two analysed AtCKX2 reduced growth all concentrations. general, showed higher than shoots. Non‐transformed tolerated up 100 without change frequency number regenerated plants. Roots different under salt stress. Regeneration ‐26 even recorded. Salinity stress response also biochemical level. Free proline, malondialdehyde hydrogen peroxide content well antioxidative enzymes activities were investigated after 1, 2, 4 8 weeks. adition culture elevated parameters roots. Considering vitro it be assumed CKs might involved defence conditions.