Serial perioperative cell‐free DNA levels in donors and recipients undergoing living donor liver transplantation

• DOI: 10.1111/aas.12947
• Publication Date: 2017-08-02T09:47:45Z
• Authors: K. Prakash; S. Aggarwal; S. Bhardwaj; G. Ramakrishna; C. K. Pandey
• Keywords: Cell-free fetal DNA

Background Effect of anaesthesia and surgery on cell‐free DNA (cf ) is not known. Given that surgical stress augments inflammation injury, we hypothesized levels cf will fluctuate during perioperative period. Therefore, in this study serial concentration was measured donors recipients undergoing living donor liver transplantation ( LDLT ). Methods Baseline, post‐induction, intraoperative post‐operative plasma were evaluated 21 each, by Sytox green method. In addition, qPCR performed a subset samples. Results Baseline higher (37.62 ng/ml) than (25.49 ng/ml). A decrease observed following induction both (11.90 (10.75 When the kinetics monitored further, an increase noted intraoperatively (46.18 (anhepatic phase: 56.25 ng/ml, reperfusion 54.36 remained high post‐operatively. One recipient who developed sepsis had highest level (94.72 Conclusion Plasma are indicative injury. Lower may be attributed to subduing effect anaesthetic agents cell death. High seen intra‐ phases reflect cellular trauma inflammation. This similar pattern fluctuation suggestive its possible utility as marker. comparable anhepatic phase less ischemia injury .