3D reconstruction of murine mitochondria reveals changes in structure during aging linked to the MICOS complex

• DOI: 10.1111/acel.14009
• Publication Date: 2023-11-14T07:13:12Z
• Authors: Zer Vue; Edgar Garza‐Lopez; Kit Neikirk; Prasanna Katti; Larry Vang; Heather Beasley; Jianqiang Shao; Andrea G. Marshall; Amber Crabtree; Alexandria C. Murphy; Brenita C. Jenkins; Praveena Prasad; Chantell Evans; Brittany Taylor; Margaret Mungai; Mason Killion; Dominique Stephens; Trace A. Christensen; Jacob Lam; Benjamin Rodriguez; Mark A. Phillips; Nastaran Daneshgar; Ho‐Jin Koh; Alice Koh; Jamaine Davis; Nina Devine; Mohammad Saleem; Estevão Scudese; Kenneth Ryan Arnold; Valeria Vanessa C...; Ryan Daniel Robinson; Moumita Chakraborty; Jennifer A. Gaddy; Mariya T. Sweetwyne; Genesis Wilson; Elma Zaganjor; James Kezos; Cristiana Dondi; Anilkumar K. Reddy; Brian Glancy; Annet Kirabo; Anita M. Quintana; Dao‐Fu Dai; Karen Ocorr; Sandra A. Murray; Steven M. Damo; Vernat Exil; Blake Riggs; Bret C. Mobley; Jose A. Gomez; Melanie R. McReyn...; Antentor Hinton
• Keywords: Bioenergetics

Abstract During aging, muscle gradually undergoes sarcopenia, the loss of function associated with mass, strength, endurance, and oxidative capacity. However, 3D structural alterations mitochondria aging in skeletal cardiac tissues are not well described. Although mitochondrial is decreased capacity, genes responsible for morphological changes during poorly characterized. We measured morphology aged murine gastrocnemius, soleus, using serial block‐face scanning electron microscopy reconstructions. also used reverse transcriptase‐quantitative PCR, transmission quantification, Seahorse analysis, metabolomics lipidomics to measure after contact site cristae organizing system (MICOS) complex genes, Chchd3 , Chchd6 Mitofilin . identified significant size tissues. found that both age‐related MICOS knockouts mice altered morphology. Given critical role maintaining cellular metabolism, we characterized metabolomes lipidomes young mouse tissues, which showed profound consistent membrane integrity, supporting our observations a relationship between aging. Thus, it important understand mechanisms underlie tissue‐dependent phenotypic occur evolutionary conservation these Drosophila mammals.