Identification of a short interspersed repetitive element insertion polymorphism in the porcineMX1promoter associated with resistance to porcine reproductive and respiratory syndrome virus infection

• DOI: 10.1111/age.12316
• Publication Date: 2015-06-20T07:26:49Z
• Authors: Yanping Li; Sen Liang; Hao Liu; Yi Sun; Li Kang; Yunliang Jiang
• Keywords: Genetic Markers; Myxovirus Resistance Proteins; 0301 basic medicine; Polymorphism, Genetic; Genotype; Swine; Molecular Sequence Data; Sus scrofa; Porcine Reproductive and Respiratory Syndrome; 3. Good health; Interspersed Repetitive Sequences; 03 medical and health sciences; Gene Frequency; Animals; Promoter Regions, Genetic; Alleles; Disease Resistance

The myxovirus resistance (Mx) proteins belong to the dynamin superfamily and are important for innate host defence against RNA viruses. In this study, we demonstrate that positive elements present in two promoter regions of −2713 −2565 −688 −431 porcine MX1 gene. Sequencing alignment amplified gene region identified a short interspersed repetitive element (SINE) insertion 275 bp at site −547. At site, allele B (an bp) is dominant Chinese indigenous pig breeds but has workable minor frequency western lean-type breeds. Luciferase activity was compared between promoters with without 275-bp fragment transiently transfected MARC-145 cells. increased luciferase significantly (P < 0.05) both prior post-porcine reproductive respiratory syndrome (PRRS) virus inoculation. These results suggest SINE polymorphism −547 potential DNA marker PRRS pigs.