Morphometric study of the testis in sheep embryos using unbiased design‐based stereology

• DOI: 10.1111/ahe.12746
• Publication Date: 2021-10-17T03:23:51Z
• Authors: Javad Sadeghinezhad; Zahra Ganji; Sirous Sadeghian ...; Hadis Bojarzadeh; Mahdi Aghabalazad...; Ali Bayat Khomejini; Ehsan Roominai; Mohsen Hosseini; Margherita De Silva
• Keywords: Stereology; Seminiferous tubule; Interstitial space; Testicular volume

Sheep have been used as translational models of human postnatal testicular development. However, the morphometric features normal developing testis in sheep embryos not previously investigated using stereology. The objective present work was to establish quantitative parameters for fetal tissue components sheep, unbiased design-based stereological methods. Twenty-four were divided into four gestational age groups (9-11, 12-14, 15-17 and 18-20 weeks gestation) on basis embryos' crown-rump length. Isotropic, systematic uniform random sections left testes obtained by employing orientator method. Testicular total volume, absolute proportional volumes occupied seminiferous tubules interstitial tissue, well tubule length, estimated point-counting system counting frame principle. All parameters, with exception tissue's fractional gradually increased along age, maximum increase especially seen late stages. volume other hand, showed a decreasing trend increasing age. testes, length significant (p< 0.05) positive linear correlation Several similarities observed embryogenesis. data emerging from study might prove useful basic contribution fields andrology embryology stimulate further research these areas.