Combining individual Chlamydia trachomatis IgG antibodies MOMP, TARP, CPAF, OMP2, and HSP60 for tubal factor infertility prediction

• DOI: 10.1111/aji.13091
• Publication Date: 2019-01-10T17:02:33Z
• Authors: Eleanne F. van Ess; Anat Eck-Hauer; Jolande A. Land; Servaas A. Morré; Sander Ouburg
• Keywords: Tubal factor infertility; Pelvic inflammatory disease

Problem Tubal factor infertility (TFI) is a severe complication of genital Chlamydia trachomatis infections. In fertility workup, chlamydia antibody test (CAT) used to predict TFI. The predictive value for TFI most commonly CAT moderate. Method study A total 183 infertile Dutch Caucasian women were included in this study. All underwent tubal patency testing (hysterosalpingography [HSG] or laparoscopy). Cases had TFI, and controls no (ie normal findings during HSG was categorized based on severity (TFI 1‐TFI 4). This investigated the values major outer membrane protein (MOMP), translocated actin‐recruiting phosphoprotein (TARP), chlamydial protease‐like activity (CPAF), heat shock protein‐60 (HSP60) 2 (OMP2) algorithm developed detect with high certainty combinations titres. Serum tested Mikrogen recomLine immunoblot quantified recomScan. greedy that explores all possible developed. Results Significant differences distributions antigen titres between cases observed CPAF ( P = 0.0021), HSP60 0.0061), MOMP 0.0497) OMP2 0.0016). Single antibodies could not discriminate by themselves. performs better specificity, positive (PPV), accuracy clinical utility index than original algorithm. combined identified 18.2% 100% certainty. Most 4 cut‐offs > 10.7 3.9. Conclusion proof‐of‐principle shows serum are commercially available can be adapted specificity.