Pollen‐derived adenosine is a necessary cofactor for ragweed allergy

• DOI: 10.1111/all.12642
• Publication Date: 2015-05-02T13:38:50Z
• Authors: M. Wimmer; F. Alessandrini; S. Gilles; U. Frank; S. Oeder; M. Hauser; J. Ring; F. Ferreira; D. Ernst; J. B. Winkler; P. Schmitt‐Kopplin; C. Ohnmacht; H. Behrendt; C. Schmidt‐Weber; C. Traidl‐Hoffmann; J. Gutermuth
• Keywords: Allergic Inflammation; Ambrosia artemisiifolia

Abstract Background Ragweed ( Ambrosia artemisiifolia ) is a strong elicitor of allergic airway inflammation with worldwide increasing prevalence. Various components ragweed pollen are thought to play role in the development responses. The aim this study was identify critical factors for allergenicity physiological model inflammation. Methods Aqueous extract, low molecular weight fraction or major allergen Amb 1 instilled intranasally on 1–11 consecutive days, and evaluated by bronchoalveolar lavage, lung histology, serology, gene expression tissue, measurement function. Pollen‐derived adenosine removed from extract enzymatically analyze its ragweed‐induced allergy. Migration human neutrophils eosinophils toward supernatants ragweed‐stimulated bronchial epithelial cells analyzed. Results Instillation but not fraction, induced specific IgG , pulmonary infiltration inflammatory cells, Th2‐associated cytokine signature impaired Adenosine aggravated In vitro migrated stimulated only if present. Conclusions factor ragweed‐pollen‐induced Future studies at therapeutic strategies control these allergen‐independent pathways.