Functional and phenotypic analysis of basophils allows determining distinct subtypes in patients with chronic urticaria

• DOI: 10.1111/all.13215
• Publication Date: 2017-06-06T03:44:53Z
• Authors: M. M. Rauber; J. Pickert; L. Holiangu; C. Möbs; W. Pfützner
• Keywords: Basophil activation

Abstract Background Chronic urticaria ( CU ) is a frequent skin disease characterized by relapsing appearance of pruritic hives. While clinical symptoms are due to the release histamine cutaneous mast cells, underlying pathophysiology still unknown. However, previous studies indicate that basophils might be relevance. Besides, occurrence autoantibodies against IgE or its receptor, Fcε RI , and therapeutic efficacy anti‐IgE antibodies imply IgE‐mediated mechanisms also play an important role in . Methods Reactivity patients’ peripheral blood (n=60) specific anti‐Fcε IgE‐independent fMLP stimulation was determined basophil activation test comparison with patients suffering from allergic rhinitis (n=10) healthy controls (n=10). In addition, immunoglobulin receptor (Fcε Fcγ RII expression surface bound (IgE, IgG) were quantified on basophils. Furthermore, autoreactive capacity sera evaluated urticaria‐related assessed both UCT ‐Q 2 oL. Results Stimulating via we identified three distinct immunologic phenotypes. One subgroup reacted stimulation, whereas others had nonreactive Among latter, pronounced basopenia identified. Of note, this group augmented serum‐induced activation, increased levels thyroid peroxidase, exhibited strongest impact their quality life. Conclusions Patients can categorized into subgroups based reactivity frequency. These phenotypes associated different characteristics, pointing as players pathophysiology.