IgG1+ B‐cell immunity predates IgE responses in epicutaneous sensitization to foods

• DOI: 10.1111/all.13481
• Publication Date: 2018-05-23T02:20:00Z
• Authors: R. Jiménez‐Saiz; Y. Ellenbogen; J. F. E. Koenig; M. E. Gordon; T. D. Walker; D. Rosace; P. Spill; K. Bruton; J. Kong; K. Monteiro; J. Wen; E. I. Tuomanen; R. Kolbeck; D. K. Chu; S. Waserman; M. Jordana
• Keywords: Memory B cell

Abstract Background The generation of IgE‐mediated food allergy in humans is silent and only diagnosed upon manifestation clinical symptoms. While experimental models have been used to investigate some mechanisms allergic sensitization, the humoral immunity memory remains be elucidated. Here, we defined evolution allergen‐specific B‐cell responses during epicutaneous sensitization foods. Methods Wild‐type genetic knockout animals, drug or antibody strategies for cell depletion immunoglobulin signaling blockade were disease progression; analyzed germinal centers IgG1 + B cells by flow cytometry, evaluated responses, determined reactivity (anaphylaxis). Results Epicutaneous caused microscopic skin damage, inflammation, recruitment activated dendritic draining lymph nodes. This process generated center cells, serum IgG1, anaphylaxis that was mediated alternative pathway. Whether peanut and/or ovalbumin from egg white compartment predominantly exhibited an immature, pro‐germinal phenotype (PDL‐2 − CD80 CD35 CD73 ). Subsequent subclinical exposures allergen induced IgE IgE, likely classical pathway anaphylaxis. Conclusions Our data demonstrate against allergens precedes responses. Therefore, assessment cellular may help identify individuals at risk developing hence provide a window therapeutic interventions.