miR‐10a‐5p is increased in atopic dermatitis and has capacity to inhibit keratinocyte proliferation

• DOI: 10.1111/all.13849
• Publication Date: 2019-05-02T17:38:14Z
• Authors: Helen Vaher; Toomas Runnel; Egon Urgard; Alar Aab; Gemma Carreras Ba...; Julia Maslovskaja; Kristi Abram; Liisi Raam; Bret Kaldvee; Tarmo Annilo; Eric R. Tkaczyk; Toivo Maimets; Cezmi A. Akdis; Külli Kingo; Ana Rebane
• Keywords: Adult; Keratinocytes; Male; Immunology; atopic eczema; 610 Medicine & health; noncoding RNA; Dermatitis, Atopic; Young Adult; 03 medical and health sciences; 10183 Swiss Institute of Allergy and Asthma Research; hyaluronic acid; Immunology and Allergy; Humans; Cell Proliferation; Skin; 2403 Immunology; 0303 health sciences; Gene Expression Profiling; Cell Cycle; Cell Differentiation; Middle Aged; allergy; MicroRNAs; Gene Expression Regulation; 2723 Immunology and Allergy; Cytokines; cell cycle; Female; RNA Interference; Disease Susceptibility

miR-10a-5p has been shown to regulate cancer cell proliferation and invasiveness endothelial inflammatory responses. The function of in the skin not previously studied. aim current study was examine expression, regulation, keratinocytes (KCs) association with atopic dermatitis (AD).The expression its target genes analyzed using RT-qPCR, mRNA array analysis, situ hybridization, immunofluorescence. transfection miRNA mimics, cycle distribution luciferase assays used functions human primary KCs.miR-10a-5p found be upregulated lesional from patients AD proliferating KCs. Array pathway analysis IL-1β-stimulated KCs revealed that inhibited many affect progression only a few inflammation-related genes. Accordingly, fewer cells S-phase reduced were detected as characteristics miR-10a-5p-transfected influence on also evident induced by AD-related cytokines, including IL-4, IL-17, IL-1β, measured capacity strongly suppress marker Ki-67. Among putative direct genes, we verified hyaluronan synthase 3, damage-associated positive regulator KC migration proliferation, miR-10a-5p.miR-10a-5p inhibits directly targets 3 thereby may modulate AD-associated processes skin.