Human airway epithelial extracellular vesicle miRNA signature is altered upon asthma development

• DOI: 10.1111/all.14008
• Publication Date: 2019-08-06T12:58:47Z
• Authors: Sabine Bartel; Stefania La Grutta; Giovanna Cilluffo; Giovanni Perconti; Antonella Bongiov...; Agata Giallongo; Jochen Behrends; Jochen Kruppa; Stefanie Hermann; Dapi Chiang; Michael W. Pfaffl; Susanne Krauss‐Et...
• Keywords: Extracellular vesicles; Basal (medicine); Exosome

miRNAs are master regulators of signaling pathways critically involved in asthma and transferred between cells extracellular vesicles (EV). We aimed to investigate whether the miRNA content EV secreted by primary normal human bronchial epithelial (NHBE) is altered upon development.NHBE were cultured at air-liquid interface treated with interleukin (IL)-13 induce an asthma-like phenotype. isolations precipitation from basal culture medium or apical surface wash characterized nanoparticle tracking analysis, transmission electron microscopy, Western blot, EV-associated identified a RT-qPCR-based profiling. Significant candidates confirmed EVs isolated size-exclusion chromatography nasal lavages children mild-to-moderate (n = 8) severe 9), healthy controls 9).NHBE secrete side. 47 expressed 16 thereof significantly IL-13 treatment. Expression could be lavages. Of note, levels miR-92b, miR-210, miR-34a correlated lung function parameters (FEV1 FVC%pred FEF25-75%pred ), thus lower sEV-miRNA associated airway obstruction. Subsequent ingenuity pathway analysis predicted regulate Th2 polarization dendritic cell maturation.Our data indicate that secretion epithelium, particular miR-34a, might early development response airways asthma.