Household laundry detergents disrupt barrier integrity and induce inflammation in mouse and human skin

• DOI: 10.1111/all.15891
• Publication Date: 2023-09-28T06:35:22Z
• Authors: Arturo O. Rinaldi; Manru Li; Elena Barletta; Paolo D'Avino; Duygu Yazici; Yagiz Pat; Siobhan Ward; Daniel Burla; Ge Tan; Nima Askary; Rasmus Larsson; Jeremy Bost; Huseyn Babayev; Raja Dhir; Nicolas Gaudenzio; Mubeccel Akdis; Kari Nadeau; Cezmi A. Akdis; Yasutaka Mitamura
• Keywords: Ex vivo; Transepidermal water loss; Barrier function; Human skin; Proinflammatory cytokine; Loricrin

Abstract Background Epithelial barrier impairment is associated with many skin and mucosal inflammatory disorders. Laundry detergents have been demonstrated to affect epithelial function in vitro using air–liquid interface cultures of human cells. Methods Back C57BL/6 mice was treated two household laundry at several dilutions. Barrier assessed by electric impedance spectroscopy (EIS) transepidermal water loss (TEWL) measurements after the 4 h treatments detergents. RNA sequencing (RNA‐seq) targeted multiplex proteomics analyses biopsy samples were performed. The 6‐h treatment effect detergent sodium dodecyl sulfate (SDS) investigated on ex vivo skin. Results Detergent‐treated showed a significant EIS reduction TEWL increase compared untreated skin, relatively higher sensitivity dose–response EIS. RNA‐seq expression genes essential for integrity, such as tight junctions adherens junction proteins. In contrast, keratinization, lipid metabolic processes, epidermal cell differentiation upregulated. Proteomics analysis that generally downregulated adhesion‐related proteins, adhesion molecule contactin‐1, upregulated proinflammatory interleukin 6 1 beta. Both SDS led decrease values model. Conclusion present study its main component, impaired Daily exposure may cause disruption contribute development atopic diseases.