Protective effect of thymoquinone against testicular torsion induced oxidative injury

• DOI: 10.1111/and.12424
• Publication Date: 2015-04-23T10:34:57Z
• Authors: M. Ayan; U. Tas; E. Sogut; S. Caylı; H. Kaya; M. Esen; F. Erdemir; M. Uysal
• Keywords: Thymoquinone; Malondialdehyde

We aimed to determine the protective effects of thymoquinone (TQ), against ischaemia–reperfusion (I/R) injury in testis tissue rats. Twenty-seven male Wistar albino rats were randomly divided into three equal groups as follows: Group I, sham group; II, torsion and III, + group. The ischaemia period was 2 h, orchiectomy performed after 30 min detorsion. Testis sections analysed with terminal transferase mediated dUTP-nick end labelling (TUNEL) assay situ apoptotic DNA fragmentation. Additionally, Caspase 3 Bax proteins immunohistochemically. superoxide dismutase (SOD), glutathione peroxidase (GSH-Px) malondialdehyde (MDA) activity levels also measured. group significantly higher than those (P < 0.05). Thymoquinone administration reduced these levels. Torsion increased active-Caspase expression, which decreased by thymoquinone. index that control However, Our results indicate plays a role oxidative stress induced