Improvement in sperm functional competence through modified low‐dose packaging in French mini straws of bull semen

• DOI: 10.1111/and.13003
• Publication Date: 2018-03-07T07:52:40Z
• Authors: P. Karan; T. K. Mohanty; A. Kumaresan; M. Bhakat; R. K. Baithalu; K. Verma; S. Kumar; M. Das Gupta; K. K. Saraf; S. C. Gahlot
• Keywords: Capacitation; Semen quality; Semen cryopreservation

To achieve the targeted artificial insemination coverage with current rate of semen production, without affecting conception rate, it needs to reduce number spermatozoa per dose in India as international practice. Therefore, this study was planned perform different levels dilution, compare vitro post-thaw quality and develop a modified low-dose packaging method French mini straw minimise dilution effect. Sixteen ejaculates were collected from Karan Fries bulls (n = 4). The mean percentage motility, viability, membrane integrity, acrosome lipid peroxidation capacitation status estimated sperm function assays sample diluted 20, 15, 10 5 million 0.25 ml filled by conventional packaging. No significant (p > .05) difference observed between 15 20 doses; however, below get reduced. There no traits spermatozoa/dose In conclusions, is very effective for cryopreservation acceptable quality.