Incubation of frozen‐thawed llama sperm with seminal plasma

• DOI: 10.1111/and.13597
• Publication Date: 2020-04-30T19:20:06Z
• Authors: Fernanda Gabriela...; Susana Marí Giuliano; Graciela Chaves; Deborah M. Neild; Marcelo Horacio M...; Mariana LucÍa Ber...; Maria Ignacia Car...
• Keywords: Semen Analysis

Seminal plasma is intimately connected to sperm physiology and particularly in South American Camelids, has demonstrated be involved multiple physiological reproductive events. Different percentages of seminal (0%, 10% 50%) were added thawed llama semen samples with the objective evaluating interaction cryopreserved over time (0, 1.5 3 hr at 37°C). A total 20 ejaculates from five adult males (n = 5; r 4) evaluated. significant decrease motility, membrane function live was observed all 0 when compared raw semen. Neither morphology nor chromatin condensation altered (p > .05), but a increase percentage spermatozoa fragmented DNA after thawing versus When time, motility observed, while preserved incubation final concentrations To conclude, addition or 50% incapable preserving frozen-thawed during incubation.