The effects of CoQ10 supplement on matrix metalloproteinases, oxidative DNA damage and pro‐inflammatory cytokines in testicular ischaemia/reperfusion injury in rats

• DOI: 10.1111/and.13839
• Publication Date: 2020-12-24T23:47:45Z
• Authors: Kemal Ayengin; Hamit Hakan Alp; Zübeyir Huyut; Serkan Yıldırım; Fikret Altındag; Veli Avci
• Keywords: Male; 0301 basic medicine; Ubiquinone; Matrix Metalloproteinases; Rats; 3. Good health; Oxidative Stress; 03 medical and health sciences; Ischemia; Malondialdehyde; Reperfusion Injury; Testis; Animals; Cytokines; Matrix Metalloproteinase 2; Rats, Wistar; Spermatic Cord Torsion

We aimed to study the effect of coenzyme Q10 on pro-inflammatory cytokine, matrix metalloproteinase, oxidative DNA damage, caspase 3 and caspase 8 in ischaemia/reperfusion injury led to by testicular torsion/detorsion. Our research is a controlled experimental animal research using rats. This study was conducted with fifty-six adult male Albino Wistar rats. Interleucine-1β, 2, 6, 10, tumour necrosis factor-α, matrix metalloproteinase-2, 3, 9, 13, tissue inhibitor matrix metalloproteinase-1, 2, malondialdehyde and leucocyte 8-hydroxy-2-deoxy guanosine/106 deoxyguanosine was detected in serum and tissue samples. In addition, immunohistochemical analysis of caspase 2 and caspase 8 was performed. In testicular I/R injury, especially 24 hr after detorsion, oxidative damage pro-inflammatory cytokines and matrix metalloproteinases were increased. At the coenzyme Q10 group, a meaningful decrease was observed in these parameters. In addition, a decrease in the expression of caspase3 and caspase 8 was viewed in coenzyme Q10-treated groups. The coenzyme Q10 has beneficial effects on oxidative damage, pro-inflammatory cytokine levels, remodelling of extracellular matrix and apoptosis in testicular I/R injury.