Effect of liver cirrhosis on erectile function in rats: A study combining bioinformatics analysis and experimental research

• DOI: 10.1111/and.14352
• Publication Date: 2021-12-18T14:24:06Z
• Authors: Jisheng Wang; Xu Cao; Sheng Deng; Bin Wang; Junlong Feng; Fanchao Meng; Hongsheng Xu; Shizhen Wang; Xiaobin Zao; Haisong Li; Yongan Ye
• Keywords: Liver Cirrhosis; Male; 0301 basic medicine; 03 medical and health sciences; Erectile Dysfunction; Nitric Oxide Synthase Type III; Penile Erection; Animals; Computational Biology; Humans; Penis; Rats

To explore the mechanism through which liver cirrhosis (LC) causes erectile dysfunction (ED). Bioinformatic analysis was used to predict potential signalling pathways in LC-induced ED, and N-nitrosodiethylamine establish a rat model of LC. H&E staining, Western blotting RT-qPCR were detect pathological tissue damage changes mRNA protein expression levels. In addition, levels sex hormones such as estradiol (E2), prolactin (PRL) testosterone (T) measured. The hypoxia-inducible factor (HIF) pathway an important our bioinformatics prediction. Pathological damages detected penile tissues rats. Compared with normal group's serum hormone levels, E2 PRL increased LC rats, while T decreased (p < 0.01). results from penis showed that endothelial nitric oxide synthase (eNOS) inducible (iNOS) both downregulated, HIF-1α upregulated group compared These data suggest hinders function histopathological by affecting HIF-1α, eNOS, iNOS, E2, T.