Evaluating the utility of the A.F. Genital System test for pathogen diagnosis in acute male urethritis
Adult
Male
A.F. genital
Urogenital System
Chlamydia trachomatis
Non-gonococcal
Polymerase Chain Reaction
Gonorrhea
Young Adult
03 medical and health sciences
0302 clinical medicine
AF genital system
Diagnosis
Gonokokal olmayan
Humans
Genitalia
A.F. Genital
Urethritis
Chlamydia Infections
Middle Aged
AF genital sistem
Non- gonococcal
AF Genital System
3. Good health
PCR
Teşhis
Üretrit
DOI:
10.1111/and.14377
Publication Date:
2022-01-22T12:13:17Z
AUTHORS (7)
ABSTRACT
This study evaluated the effectiveness of the A.F. Genital System (Liofilchem® , Italy) in detecting pathogens compared with multiplex real-time polymerase chain reaction (PCR) in men with acute urethritis. Men diagnosed as having acute urethritis between 1 April 2021 and 31 December 2021 were included. Urethral swab samples were obtained for A.F. Genital System and PCR testing in a randomly determined order. The efficacy of the A.F. Genital System was analysed by comparing the results of the two tests. The study included 83 patients (mean age 34.1 ± 11.3 years). A urethritis pathogen was detected in 69 patients (83.1%) by PCR and only 15 patients (18.1%) with the A.F. Genital System. The sensitivity of the A.F. Genital System in detecting acute urethritis pathogens was 21.7% (95% confidence interval [CI]: 13.6-32.8), and the specificity was 100% (95% CI: 78.5-100). Its sensitivity was 20% (95% CI: 7.1-45.2) in the diagnosis of gonococcal urethritis and 19.1% (95% CI: 11.2-30.4) in the diagnosis of non-gonococcal urethritis. PCR detected two or more urethritis pathogens in 9 patients (13.0%), while no polymicrobial infection was detected with the A.F. Genital System. Based on the results of multiplex real-time PCR, the A.F. Genital System had very low sensitivity in the detection of pathogens in acute male urethritis. It should be kept in mind that using this test in patients with acute urethritis may result in a high missed diagnosis rate for urethritis pathogens.
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