Light and electron microscopic study of mature spermatozoa from White Pekin duck (Anas platyrhynchos): an ultrastructural and molecular analysis

• DOI: 10.1111/andr.12130
• Publication Date: 2016-01-30T01:01:36Z
• Authors: Rakesh Kumar Majhi; Ashutosh Kumar; Manoj Yadav; Pradeep Kumar; Apratim Maity; Sunil C. Giri; Chandan Goswami
• Keywords: Anas; White (mutation)

The morphology, ultrastructure, and functions of mature avian spermatozoa have been immense commercial scientific interest for several reasons. This is mainly important in case birds poultry production, conservation, the use sperm morphometry as phylogenetic evidence. Avian share complex or no correlation with respect to testis and/or body size described before. In this work, we isolated from White Pekin duck (Anas platyrhynchos) by non-invasive methods performed immunostaining analysis well cytochemical using electron light microscopes. Here, report presence different post-translationally modified tubulin such tyrosinated-, detyrosinated-, acetylated-, polyglutamylated-, glycylated-tubulin specific regions spermatozoa. By field-emission scanning microscope, confirm acrosome-like structure at tip head. However, remains non-reactive common lectins Peanut Agglutinin (PNA) cholesterol-sensitive dyes Filipin. We that acrosomal primarily made lipid-based structures resistant 0.1% Triton X100. Confocal microscopy super resolution structured illumination study indicates nucleus bifurcated region. markers, perforatorium present head contains specialized organelles similar atypical mitochondria. propose these ultrastructural molecular parameters can be used species-specific features. mitochondria within may relevant mitochondrial inheritance heteroplasmy observed population.