Spermatogenesis associated 22 is required for DNA repair and synapsis of homologous chromosomes in mouse germ cells

• DOI: 10.1111/andr.12315
• Publication Date: 2017-03-10T15:25:18Z
• Authors: E. Hays; N. Majchrzak; V. Daniel; Z. Ferguson; S. Brown; K. Hathorne; S. La Salle
• Keywords: Synapsis; Synaptonemal complex; Null allele

Summary Analysis of the N ‐ethyl‐ ‐nitrosourea ( ENU )‐induced repro42 mutation previously identified spermatogenesis associated 22 Spata22 ) as a gene required for meiotic progression and fertility in both male female mice, but its specific contribution to process was unclear. Here, we report on novel, null allele Gt confirm requirement germ cell development. Similar mutant histological mating analyses indicate that gametogenesis is profoundly affected Gt/Gt males females, resulting infertility. Cytological examination confirms cells do not progress beyond zygonema arrest linked impairment synapsis DNA repair. SPATA distribution reveals it localizes foci with chromosomes during prophase I number peaks at zygonema; there are also more oocytes than spermatocytes. Furthermore, co‐localizes proteins involved recombination, including RAD 51, DMC 1, MLH present until mid‐pachynema, suggesting role resolution recombination intermediates. In fact, 1 20% pachynema. meiocytes localization MEIOB RPA (two known interact 22), immunoblotting corroborates production indeed decreased absence 22. Together, these data suggest meiosis mice.