Antioxidant effect of Elamipretide on bull's sperm cells during freezing/thawing process

• DOI: 10.1111/andr.12996
• Publication Date: 2021-03-06T14:58:40Z
• Authors: Alicja Kowalczyk; Ewa Czerniawska P...
• Keywords: Semen cryopreservation; Semen extender

Spermatozoa are subjected to drastic changes in temperature, ice crystal formation, and diverse types of stresses (chemical, physical, osmotic, oxidative) during the cryopreservation process, which severely compromise sperm quality fertility. In this study, we aimed investigate protective role Elamipretide bull's spermatozoa.The study included 36 healthy Simmental bulls with an average age 2 ± 0.5 years housed individually pens. Two ejaculates were collected from each bull using artificial vagina at 7 a.m. Subsequently, semen was extended animal protein-free commercial BIOXcell® extender (IMV Technologies) a final concentration 160 × 106 spermatozoa/ml, rated terms motile percentage, progressive motility, viability, morphological abnormality spermatozoa. Semen samples that showed more than 60% motility selected for experiment. The fresh then divided into five equal fractions. first fraction left control group (without Elamipretide), next added succession 0.1; 1; 5; 10 μM TFA (Trifluoroacetic) (MedChemExpress). After freezing thawing. Next assessed mitochondrial membrane potential acrosome integrity, antioxidant activity (SOD, CAT, MDA).It has been shown 5 proved be most effective tested parameters cells cryopreservation.In conclusion, addition significantly improved frozen-thawed their function. results indicate can used as cryoprotective agent protect against devastating effects oxidative stress increasing survival after cryopreservation.