The human sperm proteome—Toward a panel for male fertility testing

• DOI: 10.1111/andr.13431
• Publication Date: 2023-03-10T08:03:31Z
• Authors: Thomas Greither; Mario Dejung; Hermann M. Behre; Falk Butter; Holger Herlyn
• Keywords: Proteome; Semen Analysis

Abstract Background Although male factor accounts for 40%–50% of unintended childlessness, we are far from fully understanding the detailed causes. Usually, affected men cannot even be provided with a molecular diagnosis. Objectives We aimed at higher resolution human sperm proteome better causes infertility. were particularly interested in why reduced count decreases fertility despite many normal‐looking spermatozoa and which proteins might involved. Material methods Applying mass spectrometry analysis, qualitatively quantitatively examined proteomic profiles 76 differing fertility. Infertile had abnormal semen parameters involuntarily childless. Fertile subjects exhibited normozoospermia fathered children without medical assistance. Results discovered about 7000 coding genes proteome. These mainly known involvements cellular motility, response to stimuli, adhesion, reproduction. Numbers showing least threefold deviating abundances increased oligozoospermia ( N = 153) oligoasthenozoospermia 154) oligoasthenoteratozoospermia 368). Deregulated primarily engaged flagellar assembly fertilization, gametogenesis. Most these participated larger network infertility proteins. Discussion expose 31 displaying deviant under infertility, already before have relevance, including ACTL9, CCIN, CFAP47, CFAP65, CFAP251 (WDR66), DNAH1, SPEM1. propose 18 additional eightfold differential abundance further testing their diagnostic potential, such as C2orf16, CYLC1, SPATA31E1, SPATA31D1, SPATA48, EFHB (CFAP21), FAM161A. Conclusion Our results shed light on background dysfunctionality fewer produced syndromes it. The presented may prove useful elucidating mechanism