Differences in microbial profile of endometrial fluid and tissue samples in women with in vitro fertilization failure are driven by Lactobacillus abundance

• DOI: 10.1111/aogs.14297
• Publication Date: 2022-01-29T05:54:32Z
• Authors: Kreete Lüll; Merli Saare; Maire Peters; Ekaterina Kakhiani; Anastasia Zhdanova; Andres Salumets; Konstantin Boyarsky; Elin Org
• Keywords: Adult; Fertilization in Vitro; 3. Good health; Cohort Studies; Endometrium; Lactobacillus; 03 medical and health sciences; 0302 clinical medicine; Pregnancy; Humans; Female; Treatment Failure; Endometritis

The endometrial microbiota has been linked to several gynecological disorders, including infertility. It shown that the microbial profile of endometrium could have a role in fertilization and pregnancy outcomes. In this study we aim assess community tissue (ET) fluid (EF) samples women receiving vitro (IVF) treatment. We also search for possible associations between chronic endometritis (CE) microbiota.This was cohort involving 25 aged 28 42 years with both primary secondary infertility at least one IVF failure. ET EF sample collection carried out September 2016 November 2018. Each participants provided two types samples-tissue (50 total). A 16S rRNA sequencing performed on evaluation. CE diagnosed based CD138 immunohistochemistry where diagnosis confirmed presence or more plasma cells. Microbial profiles without were compared separately.We report no differences composition alpha diversity (pObserved = 0.07, pShannon 0.65, pInverse Simpson 0.59) patients. show abundance genus Lactobacillus influences variation beta (r2 0.34; false discovery rate [FDR] <9.9 × 10-5 ). 32% (8/25) had dominance paired these present different (pShannon 0.06, FDRweighted UniFrac 0.01). These results suggest are driven by Lactobacillus. microbiome (ie non-CE) our set patients similar.Our findings is an important factor influencing samples.