Conductive electrospun scaffolds with electrical stimulation for neural differentiation of conjunctiva mesenchymal stem cells

• DOI: 10.1111/aor.13425
• Publication Date: 2019-01-24T19:12:22Z
• Authors: Ali Rahmani; Samad Nadri; Habib Sayed Kazemi; Yousef Mortazavi; Mahdi Sojoodi
• Keywords: MTT assay; Electrospinning; Neural tissue engineering; Viability assay

Abstract An electrical stimulus is a new approach to neural differentiation of stem cells. In this work, the conjunctiva mesenchymal cells (CJMSCs) on 3D conductive fibrous scaffold silk fibroin (SF) and reduced graphene oxide (rGo) were examined. rGo (3.5% w/w) was dispersed in SF‐acid formic solution (10% w/v) nanofibrous fabricated using electrospinning method. SEM TEM microscopies used for characterization. CJMSCs cultured 2 impulse models (Current 1:115 V/m, 100‐Hz frequency current 2:115 v/m voltages, 0.1‐Hz frequency) applied 7 days. Also, effect impulses cell viability gene expression examined MTT assay qPCR analysis. Fibrous with 220 ± 20 nm diameter good dispersion nanosheets at surface nanofibers fabricated. The result showed scaffold, lower than 1. analysis confirmed that β‐tubulin (2.4‐fold P ≤ 0.026), MAP‐2 (1.48‐fold; 0.03), nestin (1.5‐fold; 0.03) genes higher compared frequency. Collectively, we proposed SF‐rGo scaffolds, as stimulation are strategies type pulses has an influence proliferation CJMSCs.