Comparison of animal and human blood for in vitro dynamic thrombogenicity testing of biomaterials

• DOI: 10.1111/aor.14366
• Publication Date: 2022-07-22T08:57:08Z
• Authors: Megan A. Jamiolko...; Mehulkumar Patel; Madelyn D. Golding; Richard A. Malina...; Qijin Lu
• Keywords: Thrombogenicity; High-density polyethylene; Human blood

To determine suitable alternatives to human blood for in vitro dynamic thrombogenicity testing of biomaterials, four different animal sources (ovine, bovine, and porcine from live donors, abattoir blood) were compared fresh blood.To account coagulability differences between individual donors species, each pool was heparinized a donor-specific concentration immediately before flow loop system. The target heparin level established using static thrombosis pre-test. For testing, whole recirculated at room temperature 1 h 200 ml/min through containing single test material. Four materials with varying thrombotic potentials investigated: latex (positive control), polytetrafluoroethylene (PTFE) (negative silicone (intermediate potential), high-density polyethylene (HDPE) (historically thromboresistant). Thrombus weight surface area coverage on the quantified, along platelet count reduction blood.While levels varied substantially 0.6 U/ml 7.0 among sources, source able differentiate thrombogenic thromboresistant PTFE HDPE (p < 0.05). However, only donor ovine bovine sensitive enough an increased response intermediate material HDPE.These results demonstrated that multiple (particularly may be when appropriate control anticoagulation are used.