Three‐dimensional reconstruction of the human skeletal muscle mitochondrial network as a tool to assess mitochondrial content and structural organization

• DOI: 10.1111/apha.12289
• Publication Date: 2014-03-29T15:42:47Z
• Authors: R. Dahl; S. Larsen; T. L. Dohlmann; K. Qvortrup; J. W. Helge; F. Dela; C. Prats
• Keywords: Bioenergetics; MFN2

Mitochondria undergo continuous changes in shape as result of complex fusion and fission processes. The physiological relevance mitochondrial dynamics is still unclear. In the field mitochondria bioenergetics, there a need tools to assess cell content. To develop method visualize networks high resolution volume.Confocal fluorescence microscopy imaging network stains human vastus lateralis single muscle fibres focused ion beam/ scanning electron (FIB/SEM) imaging, combined with 3D reconstruction was used tool analyse morphology measure fractional volume.Most type I II have tubular highly interconnected profusion mitochondria, which are thicker more structured (Fig. 1). some fibres, profission-isolated ellipsoid-shaped were observed. Mitochondrial volume significantly higher showed no correlation any investigated molecular biochemical measurements 2). Three-dimensional FIB/SEM data sets shows that subsarcolemmal physically intermyofibrillar 3).Two methods skeletal described can be investigate response life-style interventions and/or certain pathologies. Our results question classification into pools, they interconnected.