Effects of pro‐inflammatory cytokines on cannabinoid CB1 and CB2 receptors in immune cells

• DOI: 10.1111/apha.12474
• Publication Date: 2015-02-23T06:43:16Z
• Authors: L. Jean‐Gilles; M. Braitch; M. L. Latif; J. Aram; A. J. Fahey; L. J. Edwards; R. A. Robins; R. Tanasescu; P. J. Tighe; B. Gran; L. C. Showe; S. P. Alexander; V. Chapman; D. A. Kendall; C. S. Constantinescu
• Keywords: Proinflammatory cytokine

To investigate the regulation of cannabinoid receptors CB1 and CB2 on immune cells by pro-inflammatory cytokines its potential relevance to inflammatory neurological disease, multiple sclerosis (MS). signalling may be anti-inflammatory neuroprotective in neuroinflammatory diseases. Cannabinoids can suppress but effects these expression function are unknown.Immune from peripheral blood were obtained healthy volunteers patients with MS. Expression mRNA whole cells, mononuclear (PBMC) T was determined quantitative real-time polymerase chain reaction (qRT-PCR). protein flow cytometry. PBMC Western blotting for Erk1/2.Pro-inflammatory IL-1β, IL-6 TNF-α (the latter likely NF-κB dependently) upregulate human (PBMC). We also demonstrate upregulation increased MS compared controls.The levels upregulated cytokines, which explain their increase conditions including