Bestrophin-3 is differently expressed in normal and injured mouse glomerular podocytes

• DOI: 10.1111/apha.12516
• Publication Date: 2015-04-25T07:58:51Z
• Authors: V. Golubinskaya; J. Elvin; K. Ebefors; H. Gustafsson; C. Mallard; J. Nyström; H. Nilsson
• Keywords: Synaptopodin; Nestin

Bestrophins are putative calcium-activated chloride channels. Recently, cell-protective functions for Bestrophin-3 (Best3) were proposed. Best3 exists in different splice variants. We have here examined expression, alternative splicing and localization of mouse podocytes under normal conditions during endoplasmic reticulum (ER) stress.Best3 expression was determined on the mRNA level using quantitative PCR protein by immunohistochemistry Western blotting.Staining pronounced glomeruli detected cultured podocytes. did not co-localize with markers endothelial cells (CD31), podocyte foot processes (synaptopodin) or microtubules (actin). However, immunogold-based electron microscopy co-localization nestin showed presence primary cell bodies. Only two variants (both lacking exons 2 3, one also exon 6), but no full-length variant, detected. ER stress induced lipopolysaccharides vivo transiently elevated levels total its time courses. In thapsigargin, Best3, increased similar The marker C/EBP homologous (CHOP) vitro.Best3 is localized intracellularly bodies co-localized nestin. Two expressed podocytes, their differentially regulated stress.