Rapid identification of SARS‐CoV‐2 variants using stable high‐frequency mutation sites

• DOI: 10.1111/apm.13388
• Publication Date: 2024-03-16T06:16:53Z
• Authors: Yu Fu; Xiaobai He; Quan Fang; Fei Kong; Yan Zhang; Ting Fu; Liang Chen; YanXin Liu; Zhen Wang; Jianxin Lyu; Linjie Chen
• Keywords: Identification

Respiratory infectious viruses, including SARS‐CoV‐2, undergo rapid genetic evolution, resulting in diverse subtypes with complex mutations. Detecting and differentiating these pose significant challenges respiratory virus surveillance. To address challenges, we integrated ARMS‐PCR molecular beacon probes, allowing selective amplification discrimination of based on adjacent mutation sites. The method exhibited high specificity sensitivity, detecting as low 10 4 copies/mL via direct fluorescence analysis ~10 6 using real‐time PCR. Our robust detection approach offers a reliable efficient solution for monitoring evolving infections, aiding early diagnosis control measures. Further research could extend its application to other viruses optimize implementation clinical settings.